The team of Keston Bledman, Rondel Sorrillo, Emmanuel Callendar and Richard Thompson finished the race in 38.09s.
Word of their disqualification came minutes after they crossed the finish line.
According to the UK Guardian, Trinidad and Tobago's disqualification was based on rule 163.3, which is a lane violation, same as the disqualification which happened nearly two hours earlier to the T&T Men's 4x400M Relay Team as they competed in the Heats on Friday night.
The Trinidad and Tobago Olympic Committee also confirmed the disqualification on social media.
The 4x100M Relay was won by Jamaica which crossed the finish line in a time of 37.27s. Japan crossed second and USA third. However, the USA was later disqualified for an exchange zone violation, making 4th place finisher, Canada, the Bronze Medal winner.
The race marked what is expected to be the last Olympic appearance for legendary sprinter Usain Bolt, who ran himself into history by completing what is known as the ‘triple triple’ by becoming the only person to win Gold Medals in three consecutive Olympics.
He's won Gold in the 100M, the 200M and 4x100M Relays in the Olympic Games in Beijing in 2008, London in 2012 and now Rio de Janeiro in 2016.